Rabbit anti-CRM1 Antibody, Affinity Purified

Name	Rabbit anti-CRM1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0109
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Ape, Chimpanzee, Marmoset, Human, Mouse, Monkey, Primate
Gene	XPO1
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-CRM1 Antibody, Affinity Purified: <B>Cat. No. PLA0109</B>: Detection of Human CRM1 by Immunohistochemistry. Sample: FFPE section of Human metastatic lymph node. Antibody: Affinity purified Rabbit Anti-CRM1 (<B>Cat. No. PLA0109</B>) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-CRM1 Antibody, Affinity Purified: Cat. No. PLA0109: Detection of Human CRM1 by Immunohistochemistry. Sample: FFPE section of Human metastatic lymph node. Antibody: Affinity purified Rabbit Anti-CRM1 (Cat. No. PLA0109) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-CRM1 Antibody, Affinity Purified: <B>Cat. No. PLA0109</B>: Detection of CRM1 (Exportin 1) by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 μg), Ramos (50 μg) and Mouse NIH3T3 (50 μg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CRM1 Antibody (<B>Cat. No. PLA0109</B>) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. CRM1 was also immunoprecipitated by Rabbit Anti-CRM1 Antibodies which recognize upstream epitopes on CRM1. Detection: Chemiluminescence with an exposure time of 3 seconds (A and B).

Immunoblotting
Rabbit Anti-CRM1 Antibody, Affinity Purified: Cat. No. PLA0109: Detection of CRM1 (Exportin 1) by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 μg), Ramos (50 μg) and Mouse NIH3T3 (50 μg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CRM1 Antibody (Cat. No. PLA0109) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. CRM1 was also immunoprecipitated by Rabbit Anti-CRM1 Antibodies which recognize upstream epitopes on CRM1. Detection: Chemiluminescence with an exposure time of 3 seconds (A and B).