Rabbit anti-Phospho RPA32 (S33) Antibody, Affinity Purified

Name	Rabbit anti-Phospho RPA32 (S33) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0310
Prices	$345.00
Sizes	100 µl
Applications	PLA
Species Reactivities	Primate, Monkey, Ape, Mouse, Chimpanzee, Human
Gene	RPA2
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho RPA32 (S33) Antibody, Affinity Purified: <B>Cat. No. PLA0310</B>: Detection of Human phospho RPA32-S33 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells that were either treated with 100 μM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-phospho RPA32-S33 Antibody <B>PLA0310</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows Westen Blotting for total RPA32 using affinity purified Rabbit Anti-RPA32 Antibody <B>PLA0070</B> at 0.1 μg/mL with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-Phospho RPA32 (S33) Antibody, Affinity Purified: Cat. No. PLA0310: Detection of Human phospho RPA32-S33 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells that were either treated with 100 μM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-phospho RPA32-S33 Antibody PLA0310 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows Westen Blotting for total RPA32 using affinity purified Rabbit Anti-RPA32 Antibody PLA0070 at 0.1 μg/mL with an exposure time of 10 seconds.