IL-1a (Interleukin-1 alpha) Human SimpleStep ELISA™ Kit
| Name | IL-1a (Interleukin-1 alpha) Human SimpleStep ELISA™ Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab178008 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Cell culture supernatant, Serum, Plasma, Adherent cells, Suspension cells |
| Detection | Colorimetric |
| Sensitivity | 0.5 pg/ml |
| Range | 3 pg/ml - 200 pg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | IL1A |
| Supplier Page | Shop |
Product images
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
Human PBMC were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37ºC in the presence or absence of PHA. The concentrations of IL-1a were interpolated from the calibration curve and corrected for sample dilution. The mean IL-1a concentration was determined to be 1.4 pg/mL in unstimulated PBMC supernatants and 96 pg/mL in stimulated PBMCs.