Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-CDC25a Antibody, Affinity Purified:
Cat. No. PLA0021: Detection of Human CDC25a by Immunoprecipitation followed by Western Blot. Sample: Nuclear extract (1 mg) from HeLa cells: Antibodies: Affinity purified Rabbit Anti-CDC25a
PLA0021 used at 2 μg/mg lysate for IP and 1 μg/mL for Western Blot. Detection: Chemiluminescence.