Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Phospho Chk1 (S317) Antibody, Affinity Purified:
Cat. No. PLA0056: Detection of Human phospho Chk1 by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells treated with hydroxyurea (HU), ionizing radiation (IR), or untreated. Antibody: Affinity purified Rabbit Anti-Chk1 pSer317 (
Cat. No. PLA0056) used at 1.0 μg/mL. Detection: Chemiluminescence.