Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-Chk1 Antibody, Affinity Purified:
Cat. No. PLA0055: Detection of Human Chk1 by Western Blot and Immunoprecipitation. Sample: Whole Cell lysate (50 μg for WB, 500 μg for IP) from HeLa cells. Antibodies: Affinity purified goat Anti-Chk1 (Cat No.
PLA0055) used as indicated. Immunoprecipitated material was detected by WB using a monoclonal Antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.