Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-ATM Antibody, Affinity Purified:
Cat. No. PLA0045: Detection of Human and Mouse ATM by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (100 μg) from L-40 Human (Lane WT) or AT-59 Human (Lane AT) cells for WB. Whole cell lysate (0.5 mg) from L-40, AT-59, AT29 Mouse (Lane WT) or AP24 Mouse (AT; ATM -/-) cells for IP. Antibody: Affinity purified goat Anti-ATM (
Cat. No. PLA0045) used at 0.2 μg/mL for WB and 20 μg/mg lysate for IP. Detection: Chemiluminescence.