Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-GRLF1/p190RhoGAP Antibody, Affinity Purified:
Cat. No. PLA0210: Detection of Mouse GRLF1/p190RhoGAP by Western Blot. Samples: Whole cell lysate (50 μg) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified Rabbit Anti-GRLF1/p190RhoGAP Antibody
PLA0210 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.