Rabbit anti-p400 Antibody, Affinity Purified

Name	Rabbit anti-p400 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0116
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Horse, Pig, Monkey, Marmoset, Rat, Bovine, Human, Mouse, Primate, Chimpanzee, Guinea Pig, Ape
Gene	EP400
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-p400 Antibody, Affinity Purified: <B>Cat. No. PLA0116</B>: Detection of Human p400 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP reaction loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-p400 Antibody (<B>Cat. No. PLA0116</B>) used at 0.04 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. Immunoprecipitation of p400 was also accomplished using a Rabbit Anti-p400 Antibody which is to an upstream epitope on p400. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunoblotting
Rabbit Anti-p400 Antibody, Affinity Purified: Cat. No. PLA0116: Detection of Human p400 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP reaction loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-p400 Antibody (Cat. No. PLA0116) used at 0.04 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. Immunoprecipitation of p400 was also accomplished using a Rabbit Anti-p400 Antibody which is to an upstream epitope on p400. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

<B>Immunoblotting</B><BR/>Rabbit Anti-p400 Antibody, Affinity Purified: <B>Cat. No. PLA0116</B>: Detection of Mouse p400 by Western Blot. Samples: Whole cell lysate from CT26.WT (15 and 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-p400 Antibody <B>PLA0116</B> used for WB at 0.2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-p400 Antibody, Affinity Purified: Cat. No. PLA0116: Detection of Mouse p400 by Western Blot. Samples: Whole cell lysate from CT26.WT (15 and 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-p400 Antibody PLA0116 used for WB at 0.2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.