Rabbit anti-Claspin Antibody, Affinity Purified

Name	Rabbit anti-Claspin Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0075
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Ape, Marmoset, Human, Chimpanzee, Rat, Primate
Gene	CLSPN
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Claspin Antibody, Affinity Purified: <B>Cat. No. PLA0075</B>: Detection of Human Claspin by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-Claspin (<B>Cat. No. PLA0075</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Claspin Antibody, Affinity Purified: Cat. No. PLA0075: Detection of Human Claspin by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-Claspin (Cat. No. PLA0075) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Claspin Antibody, Affinity Purified: <B>Cat. No. PLA0075</B>: Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-Claspin Antibody <B>PLA0075</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-Claspin Antibody, Affinity Purified: Cat. No. PLA0075: Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-Claspin Antibody PLA0075 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Claspin Antibody, Affinity Purified: <B>Cat. No. PLA0075</B>: Detection of Human Claspin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-Claspin antibody <B>Cat. No. PLA0075</B> used for IP at 6 μg/mg lysate. For blotting immunoprecipitated Claspin, <B>PLA0075</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation
Rabbit Anti-Claspin Antibody, Affinity Purified: Cat. No. PLA0075: Detection of Human Claspin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-Claspin antibody Cat. No. PLA0075 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated Claspin, PLA0075 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.