IL-2 (Interleukin-2) Human SimpleStep ELISA™ Kit
| Name | IL-2 (Interleukin-2) Human SimpleStep ELISA™ Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab174444 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Cell culture supernatant, Serum, Plasma |
| Detection | Colorimetric |
| Sensitivity | 9 pg/ml |
| Range | 15.63 pg/ml - 2000 pg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | IL2 |
| Supplier Page | Shop |
Product images
Example IL-2 standard curve in sample diluents NS.
Background-subtracted data values (mean +/- SD) are graphed.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2mM L-glutamine, 100U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of IL-2 were interpolated from the calibration curve and corrected for sample dilution. The mean IL-2 concentration was determined to be 16 pg/mL in unstimulated PBMC supernatants and 11,460 pg/mL in stimulated PBMC supernatants.