
Background-subtracted data values (mean +/- SD) are graphed.

PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IL-8 concentrations were measured in serially diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background.

Interpolated concentrations of secreted IL-8 in media, unstimulated and PHA-stimulated Human PBMC. The mean IL-8 concentration was determined to be 3.28 ng/mL in unstimulated PBMC supernatants and 73.66 ng/mL in stimulated PBMC supernatants.