Figure 1: Phospho-Akt1 (Ser473) is detected in untreated Jurkat cells, where as no signal is detected with treatment with LY294002 , a specific PI3 Kinase inhibitor using PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160.
Figure 2: Treated and untreated NIH/3T3 lysates are assayed on #7160 Phospho-Akt1 (Ser 473) and #7170, Total Akt1 Sandwich ELISA kits. Total Akt1 levels can be detected in both treated and untreated lysates. Phospho-Akt1 (Ser 473) is detected only in the PDGF treated cells. The corresponding Western blots probing with #4058 and #9272 are shown in the bottom figure.
Figure 3: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and Absorbance at 450 nm is shown. Cells (80% confluence) were treated with PDGF (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes.