Using the JNK 1/2/3 assay kits, a significant stimulation of JNK 1/2/3 phosphorylation at Thr183/Tyr185 is detected in HEK293 cells treated with anisomycin for 30 minutes compared with untreated cells, while no change in total JNK levels is observed.
A Dilution series of recombinant active JNK1alpha was prepared by diluting to various concentrations in Lysis Mix containing 0.1% BSA. This dilution series was analyzed using the phospho-JNK assay. Limit of detection of active rhJNK was around 50 ng/mL.
PhosphoTracer JNK (pT183/Y185) + total JNK ELISA Kit (ab119663) used in Sandwich ELISA.