KGF (FGF7) Human SimpleStep ELISA™ Kit
| Name | KGF (FGF7) Human SimpleStep ELISA™ Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab183362 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Serum, Cell culture media, Heparin Plasma, EDTA Plasma, Citrate Plasma |
| Detection | Colorimetric |
| Sensitivity | 7.2 pg/ml |
| Range | 31.25 pg/ml - 2000 pg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | FGF7 |
| Supplier Page | Shop |
Product images
Figure 1. Example of KGF standard curve in sample diluent 25BS. The KGF standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Figure 2. Specificity of KGF signal on stimulated and non stimulated media supernatants. Human Dermal Neonatal Fibroblasts cells were cultured in HGDMEM supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37°C in the presence or absence of 1 µg/mL of FGF-basic with or without 10 ng/mL of TNF-alpha. The concentrations of KGF were measured after loading neat media on the plate and were then interpolated from a KGF calibration curve on sample diluent 25BS. The mean concentration of KGF (FGF-7) was determined to be undetectable in non stimulated media, 413 pg/mL in FGF-basic stimulated media and 1,016 pg/mL in FGF-basic+TNF-alpha stimulated media.