MIF in THP1 supernatants from control cells or cells stimulated (P/L) for 24 hours with 50 ng x mL-1 PMA (ab120297) and 1 ug x mL-1 LPS for the last 6 hours.
MIF measured in cell culture supernatants from control or treated (PHA: 2 days in 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).
MIF in human biological fluids (duplicates +/- SD). Mouse serum gave 0.12 ng/mL (duplicates) and no signal was detected in mouse plasma.
Standard curve in assay buffer B with background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab100594.
Representative Standard Curve using ab100594.