Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
Background subtracted data from duplicate measurements are plotted.
Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous MIP-1a levels. MIP-1a levels were interpolated from the protein standard curve and normalized for sample dilution. The mean MIP-1a levels were 33.9 ng/mL in PBMC + PHA conditioned media and 17.2 ng/mL in PBMC - PHA conditioned media.