Background-subtracted data values (mean +/- SD) are graphed.
RAW264.7 cells were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. MIP1a was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the MIP1a standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 20 ng/mL unstimulated, 118 ng/mL stimulated, and undetectable in media.
The concentrations of MIP1a were measured in duplicate and interpolated from the MIP1a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 118 µg/mL in RAW264.7 LPS Stimulated supernatant.