NADH Dehydrogenase (Complex I) Profiling ELISA Kit
| Name | NADH Dehydrogenase (Complex I) Profiling ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab124535 |
| Category | ELISA Kit |
| Assay Type | Sandwich (qualitative) |
| Sample Type | Tissue Extracts, Cell Lysate |
| Detection | Colorimetric |
| Sensitivity | 20 µg/ml |
| Range | 3 µg/ml - 1000 µg/ml |
| Format | Microplate (12 x 8 well strips) |
| Applications | ELISA |
| Species Reactivities | Bovine, Human |
| Supplier Page | Shop |
Product images
Example control sample curve of serially titrated HepG2 cell extracts in the working range of the assay.
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
Human HepG2 cells were cultured in chloramphenical for 6 days to ensure a significant effect on mitochondrial protein translation. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex III.