Using Western blot, a significant stimulation of p38 MAPKalpha phosphorylation at Thr180/Tyr182 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total p38 MAPKalpha levels is observed.
Using the p38 MAPKalpha assay kits, a significant stimulation of p38 MAPKalpha phosphorylation at Thr180/Tyr182 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total p38 MAPKalpha levels is observed.
PhosphoTracer p38 MAPKalpha Total ELISA Kit (ab119639) used in Sandwich ELISA.