A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126456.
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed using ab126456.
A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 10 min. Cell lysates were analyzed by Western Blot.
A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed using ab126456.
The A431 cells were treated with 100 ng/mL recombinant Human EGF for 20 minutes to induce phosphorylation of EGF R. Serial dilutions of lysates were analyzed by western blot. Immunoblots were incubated with anti-phospho-STAT1 (Tyr701).