Example of a typical STAT5 A/B (pY694/699) cell lysate dilution series.. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1 x Cell Extraction Buffer PTR. Data from duplicate measurements of STAT5 A/B (pY694/699) are normalized and plotted.
Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment. A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT5 A/B (pY694/699) are plotted. Comparative STAT5 A/B (pY694/699) and STAT5 A/B (Total) data also shown by Western Blot.