
Background-subtracted data values (mean +/- SD) are graphed.

Background subtracted data from duplicate measurements are plotted.

Background subtracted data from duplicate measurements are plotted.

Interpolated values were multiplied by their dilution factor (1:50) to represent the level in a neat sample. Triplicate measurements are shown.

Interpolated values from four concentrations were multiplied by their dilution factor to represent the level in a neat sample (Human serum mean = 975.9 ng/mL, Human plasma mean = 825.9 ng/mL).