MEF cells were treated with (lane 2) or without EGF (lane 1).Top Panel: Cell lysates were incubated with an anti-active Cdc42 monoclonal antibody. The precipitated active Cdc42 was immunoblotted with the anti-Cdc42 rabbit polyclonal antibody.Bottom Panel: Western blot of the cell lysates used (5% of that used in the top panel) with the anti-Cdc42 rabbit polyclonal antibody provided in the kit.