| Description |
Anti-Insulin Receptor Antibody (AIRA) BioAssay ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Insulin Receptor Antibody (AIRA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Insulin Receptor Antibody (AIRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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