Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476) (duplicates +/- SD).
Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476) (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Histone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.
Figure 1. Illustrated standard curve generated with H4 standard.