Malate Dehydrogenase 1 (MDH1) Activity Assay
| Name | Malate Dehydrogenase 1 (MDH1) Activity Assay |
|---|---|
| Supplier | Abcam |
| Catalog | ab200009 |
| Category | Cellular Assay |
| Prices | $470.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Quantitative |
| Sample Type | Cell culture extracts, Tissue Extracts |
| Detection | Colorimetric |
| Format | Microplate (12 x 8 well strips) |
| Applications | FA |
| Species Reactivities | Mouse, Rat, Human |
| Gene | MDH1 |
| Supplier Page | Shop |
Product images
Once the rate/slope of each lane is extracted from the linear range of the time point data, it is expressed as rate (mOD/min) per microgram of cell lysate added per well.
Representative background-subtracted kinetic measurements from serially diluted HeLa cell lysates and tissue homogenates HLH, MLH, RLH.
The assay was used to determine the MDH1 activity in a series of normal cell lysates and tissue homogenates loaded at 250 µg/mL.
MDH1 activity in serially titrated HeLa cell fractions (Cyto = Cytoplasmic, Mito = Mitochondrial and Nuc = Nuclear) demonstrates the assay’s specificity to MDH1.