Akt Kinase Assay Kit (Nonradioactive)
| Name | Akt Kinase Assay Kit (Nonradioactive) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9840 |
| Category | Assay Kit |
| Prices | $379.00 |
| Sizes | 40 assays |
| Species Reactivities | Human, Mouse, Rat |
| Description | Nonradioactive Akt Kinase Assay Kit provides all the reagents necessary to measure Akt kinase activity in the cell |
| Gene | AKT1 |
| Supplier Page | Shop |
Product images
AKT Kinase activity of PDGF-treated NIH/3T3 cell extracts was analyzed by IP/Kinase assay. Cell extracts (200 μl) were incubated overnight with Immobilized Phospho-Akt (Ser473) (D9E) Rabbit mAb #4070. After extensive washing the kinase reaction was performed in the presence of 200 μM of cold ATP and 1 μg of GSK-substrate. Phosphorylation of GSK-3 was measured by Western blot using Phospho-GSK-3 a/β (Ser21/9) Antibody #9327.
Product References
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CD40-dependent activation of phosphatidylinositol 3-kinase/Akt pathway mediates - CD40-dependent activation of phosphatidylinositol 3-kinase/Akt pathway mediates
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Jost M, Huggett TM, Kari C, Boise LH, Rodeck U. J Biol Chem. 2001 Mar 2;276(9):6320-6. Epub 2000 Nov 29.
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Kim S, Jin J, Kunapuli SP. J Biol Chem. 2004 Feb 6;279(6):4186-95. Epub 2003 Nov 17.
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Cavin LG, Wang F, Factor VM, Kaur S, Venkatraman M, Thorgeirsson SS, Arsura M. Mol Cancer Res. 2005 Jul;3(7):403-12.
PKB/AKT and ERK regulation of caspase-mediated apoptosis by methylseleninic acid - PKB/AKT and ERK regulation of caspase-mediated apoptosis by methylseleninic acid
Hu H, Jiang C, Li G, Lu J. Carcinogenesis. 2005 Aug;26(8):1374-81. Epub 2005 Apr 21.