Figure 1: CD44 protein from HeLa, PANC-1 and U-118 MG cells was detected using the PathScan® Total CD44 Sandwich ELISA Kit #13819. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using CD44 (8E2) Mouse mAb #5640 is shown in the bottom figure.
Figure 2: The relationship between protein concentration of lysates from untreated and LPS-treated THP-1 cells (1 μg/ml, 24 hr) and the absorbance at 450 nm as detected by the PathScan® Total CD44 Sandwich ELISA Kit is shown.