Compound panel (5 µM each compound): DELFIA® data were generated using HTScan® Profiling Kit (Tyrosine Kinase Set I) to detect the inhibition of various RTKs by a panel of compounds (Staurosporine, Iressa, SU-6668 and CT53518). In a 50 µl reaction, 10 Units of each RTK, 1.5 uM substrate peptide, 20 uM ATP and 5 uM inhibitor were used per reaction well (in duplicate) at 25ºC for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
Dose response (SU-6668): DELFIA® data were generated using HTScan® Profiling Kit (Tyrosine Kinase Set I) to detect the inhibition profile of SU-6668 toward various RTKs. In a 50 ul reaction, 10 Units of each GST-RTK, 1.5 uM substrate peptide, 20 uM ATP and various concentrations of SU6668 were used per reaction well at 25ºC for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
Assay plate (96 well format): Peptide substrates for the indicated eight RTKs have been arrayed in 96-well format.