Kinase of interest is incubated with biotinylated substrate peptides. Phosphorylated products are captured on streptavidin-coated plates. Cell Signaling Technology, Inc. antibody #9411 P-Tyr-100 serves as the primary detection reagent; DELFIA® Europium (Eu) anti-mouse chelates are used as secondary antibodies. Positive hits from Tyrosine Kinase Substrate Screening Kit #7450 can be used in any subsequent peptide based kinase assay.
Treatment of Tyrosine Kinase Substrate Screening Kit with Met kinase: Positive control is shown in yellow, and negative control is shown in blue. Several positive hits were identified using this approach (in red).
Treatment of Tyrosine Kinase Substrate Screening Kit with Src kinase: Positive control is shown in yellow, and negative control is shown in blue. Several positive hits were identified using this approach (in red).