PathScan ® Phospho-Chk2 (Thr68) Sandwich ELISA Kit
| Name | PathScan ® Phospho-Chk2 (Thr68) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7037 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | The PathScan ® Phospho-Chk2 (Thr68) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Chk2 when phosphorylated at Thr68 |
| Gene | CHEK2 |
| Supplier Page | Shop |
Product images
Figure 2. The relationship between the protein concentration of lysates from untreated and UV-treated HeLa cells and the absorbance at 450 nm using the PathScan ® Phospho-Chk2 (Thr68) Sandwich ELISA Kit #7037 is shown.
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of Chk2 at Thr68, detected by the PathScan ® Phospho-Chk2 (Thr68) Sandwich ELISA Kit #7037, but does not affect the levels of total Chk2 detected by PathScan ® Total Chk2 Sandwich ELISA Kit #7045. HeLa cells (80-90% confluent) were treated with 100 mJ/cm2 UV with 1 hour recovery at 37º C. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Chk2 (1C12) Mouse mAb #3440 (left panel) or Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197 (right panel) are shown in the bottom figure.