| Description |
The RING-finger ubiquitin E3 ligase Mdm2/HDM2 (Murine Double Minute 2) is an important regulator of the tumor suppressor protein p53. Mdm2/HDM2 ligase activity is at least partially responsible for the ubiquitination and subsequent proteasomal degradation of p53 and other proteins. This kit is designed for in vitro Mdm2/HDM2-mediated ubiquitination of user-supplied substrates such as p53 (SP-450), β-Arrestin, and DYRK2. Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein. A control substrate, S5a (SP-400) and detection antibody (AF5540) are included in the kit.
|