Figure 2. The relationship between the protein concentration of lysates from CCD-1070Sk cells, serum starved or hIL-1β-treated, and the absorbance at 450 nm is shown. CCD-1070Sk cells (80-90% confluent) were serum starved or treated with hIL-1β #8900 (5 ng/ml, 16 hr) and then lysed.
Figure 1. Treatment of CCD-1070Sk cells with IL-1β stimulates expression of Cox2 as detected by the PathScan ® Total Cox2 Sandwich ELISA Kit. CCD-1070Sk cells (80-90% confluent) were serum starved or treated with hIL-1β #8900 (5 ng/ml, 16 hr). The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Cox2 Antibody #4842 is shown in the bottom figure.