AKT 1/2/3 (pThr308/pSer473) + AKT1 (Total) PhosphoTracer ELISA Kit
| Name | AKT 1/2/3 (pThr308/pSer473) + AKT1 (Total) PhosphoTracer ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab119675 |
| Category | ELISA Kit |
| Assay Type | Semi-quantitative |
| Sample Type | Cell culture extracts |
| Detection | Fluorescent |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Mouse, Hamster, Human, Rat |
| Gene | AKT1 |
| Supplier Page | Shop |
Product images
Using the AKT 1/2/3 (p-Thr308) assay kits or Western blot, AKT 1/2/3 phosphorylation at Thr308 is detected in serum-treated HEK293 cells (+), compared with cells treated with 2 µM wortmannin (-), whereas no change in total AKT 1/2/3 levels was observed.
Using the AKT 1/2/3 assay kits, a significant increase in AKT 1/2/3 phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-), whereas no change in total AKT 1/2/3 levels was observed.