PathScan ® Total Cyclin D1 Sandwich ELISA Kit
| Name | PathScan ® Total Cyclin D1 Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7918 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Description | The PathScan ® Total Cyclin D1 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total cyclin D1 protein |
| Gene | CCND1 |
| Supplier Page | Shop |
Product images
Figure 1. Cyclin D1 protein from human (HT-1080 and MCF7), monkey (COS-7), mouse (C2C12), and rat (C6) cells was detected using PathScan ® Total Cyclin D1 Sandwich ELISA Kit #7918. However, this kit has a low detection level for cyclin D1 in some cell lines, such as HeLa, Mv 1 Lu or NIH/3T3. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Cyclin D1 Antibody #2922, is shown in the bottom figure.
Figure 2. The relationship between protein concentration of lysates from HT-1080 cells and the absorbance at 450 nm is shown. HT-1080 cells (80% confluence) were harvested and then lysed.