HeLa cells were treated with Anisomycin. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
HeLa cells were treated or untreated with Anisomycin. Cell lysates were analyzed using this phosphoELISA and Western Blot.
HeLa cells were treated or untreated with Anisomycin. Cell lysates were analyzed using this phosphoELISA and Western Blot.