Jurkat cells were treated with Pervanadate at 37°C for 10 min. Solubilize cells at 4 x 10^7 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
U251 cells were treated or untreated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA: