T47D cells were irradiated with UV. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
T47D cells were treated or untreated with UV. Cell lysates were analyzed using this phosphoELISA and Western Blot.
T47D cells were treated or untreated with UV. Cell lysates were analyzed using this phosphoELISA and Western Blot.