Jurkat cells were treated with H2O2 at 37°C for 2 min. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
Jurkat cells were treated or untreated with 2mM H2O2 for 2 min. Cell lysates were analyzed using this phosphoELISA.
Jurkat cells were treated or untreated with 2mM H2O2 for 2 min. Cell lysates were analyzed using this Western Blot.