JURKAT cells were treated with Pervanadate at 37°C for 10 min. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
JURKAT cells were treated or untreated with Pervanadate for 10 min. Cell lysates were analyzed using this phosphoELISA.