Figure 1. Treatment of A-431 cells with hEGF #8916 stimulates tyrosine phosphorylation of EGF receptor detected by PathScan ® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit #7911, but does not affect the level of total EGF receptor detected by PathScan ® Total EGF Receptor Sandwich ELISA Kit #7250. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using EGF Receptor Antibody #2232 (left panel) or Phospho-EGF Receptor (Tyr1173) (53A2) Rabbit mAb #4407 (right panel) are shown in the bottom figure.
Figure 2. The relationship between protein concentration of lysates from untreated and EGF-treated A-431 cells and the absorbance at 450 nm is shown. After starvation, A-431 cells (85% confluence) were treated with hEGF #8916 (100 ng/ml) for 5 min at 37°C and then lysed.