Figure 2. The relationship between the protein concentration of lysates from Jurkat cells, treated with Calyculin A #9902 and pervanadate or LY294002, and the absorbance at 450 nm using PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit is shown.
Figure 1. Treatment of Jurkat cells with 100 nM Calyculin A #9902 and 1 mM pervanadate increases phosphorylation of eIF2α at Ser51, detected by the PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit #7286, but does not affect the levels of total eIF2α detected by PathScan ® Total eIF2α Sandwich ELISA Kit #7952. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using eIF2α (L57A5) Mouse mAb #2103 (left panel) or Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 (right panel) are shown in the bottom figure.