PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit
| Name | PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7938 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | The PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eIF4E when phosphorylated at Ser209 |
| Gene | EIF4E |
| Supplier Page | Shop |
Product images
Figure 1. Treatment of serum-starved HeLa cells with 20% FBS stimulates phosphorylation of eIF4E at Ser209, detected by the PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit #7938, but does not affect the levels of total eIF4E detected by PathScan ® Total eIF4E Sandwich ELISA Kit #7940. HeLa cells (80-90% confluent) were serum-starved over night and then treated with 20% FBS at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using eIF4E Antibody #9742 (left panel) or Phospho-eIF4E Antibody (Ser209) #9741 (right panel) are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of lysates from untreated and 20% FBS-treated HeLa cells and the absorbance at 450 nm using the PathScan ® Phospho-eIF4E (Ser209) Sandwich ELISA Kit is shown.