PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
| Name | PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7177 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse |
| Description | The PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of p44/42 MAPK when dually phosphorylated at Thr202/Tyr204 of p44 (Thr185/Tyr187 of p42), and singly phosphorylated at Thr202 of p44 (Thr185 of p42) |
| Gene | MAPK3 |
| Supplier Page | Shop |
Product images
Figure 1. Treatment of NIH/3T3 cells with PDGF stimulates phosphorylation of p44/42 MAPK at Thr202/Tyr204, detected by the PathScan ® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit #7177, but does not affect the level of p44/42 MAPK detected by western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots, using p44/42 MAPK (Erk1/2) Antibody #9102 (left) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb #4370 (right), are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of the lysate from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm is shown.