Catalase Human ELISA Kit
| Name | Catalase Human ELISA Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab123456 |
| Category | ELISA Kit |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Tissue Extracts, Cell Lysate |
| Detection | Colorimetric |
| Sensitivity | 4 ng/ml |
| Range | Sample specific recoverySample typeAverage %RangeSerum102101% - 104% |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Gene | CAT |
| Supplier Page | Shop |
Product images
Example standard curve. Serially diluted recombinant Catalase in the working range of the assay.
Serially titrated HeLa extract in the assay working range.
Immunocytochemistry image of Human HeLa cells stained with the antibody from this kit. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the detector antibody (12C2DB9, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates in the peroxisomes.