PathScan ® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit
| Name | PathScan ® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7996 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse |
| Description | The PathScan ® Total Acetyl-CoA Carboxylase (ACC) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ACC. An ACC mouse antibody has been coated onto the microwells |
| Gene | ACACA |
| Supplier Page | Shop |
Product images
Figure 2. The relationship between the protein concentration of lysates from untreated and H 2 O 2 -treated Hep G2 cells and the absorbance at 450 nm using the PathScan ® Total ACC Sandwich ELISA Kit #7996 is shown.
Figure 1. Treatment of Hep G2 cells with H 2 O 2 stimulates phosphorylation of ACC at Ser79, detected by the PathScan ® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986, but does not affect the levels of total ACC detected by PathScan ® Total ACC Sandwich ELISA Kit #7996. Hep G2 cells (80-90% confluent) were treated 10 mM hydrogen peroxide for 10 minutes and lysed with #7018. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (left panel) or Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 (right panel) are shown in the bottom figure.