Background-subtracted data values (mean +/- SD) are graphed.
Mean human Clusterin is 63 µg/mL and values fall within expected normal reference ranges.
Background subtracted data from triplicate measurements are plotted.
HeLa cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous Clusterin levels. Background subtracted data from triplicate measurements are plotted. Mean calculated value for endogenous Clusterin was 231 ng/mL.