Bisindolylmaleimide I, Hydrochloride
| Name | Bisindolylmaleimide I, Hydrochloride |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9841 |
| Prices | $79.00 |
| Sizes | 500 µg |
| Formula | C25H24N4O2•HCl |
| Molecular Weight | 448.95 g/mol |
| Purity | >99% |
| Description | Bisindolylmaleimide I (BIS) is a potent inhibitor of PKC (1,2). In vitro the IC 50 of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC 50 for PKCζ is about 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays the IC 50 of BIS for PKC is between 0.2-2 μM (1,3). |
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Product images
Western blot analysis of extracts from A172 cells, untreated or treated with indicated concentrations of Bisindolylmaleimide I for 30 minutes, followed by stimulation with 200 nM TPA for 10 minutes. The phosphorylation of PEA-15 was detected by using Phospho-PEA-15 (Ser104) Antibody #2776 (upper). PEA-15 Antibody #2780 was used as a loading control (lower). Bisindolylmaleimide I-mediated inhibition of TPA-induced PKC phosphorylation of PEA-15 at Ser104 is shown.
Bisindolylmaleimide I structure