Brefeldin A
| Name | Brefeldin A |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 9972 |
| Prices | $99.00 |
| Sizes | 5 mg |
| Formula | C16H24O4 |
| Molecular Weight | 280.4 g/mol |
| Purity | >99% |
| Description | Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulum to the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7). |
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Product images
Confocal immunofluorescent analysis of MCF-7 cells, untreated (left) or treated with Brefeldin A for 1.5 hours at 100 ng/ml (middle) or 20 μg/ml (right), using a Golgin-97 antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).